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martine roussel  (Addgene inc)


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    Structured Review

    Addgene inc martine roussel
    Martine Roussel, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 18 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/martine+roussel/pCDNA3-HA-HA-humanCMYC+(Plasmid+%2374164)/pmc12288966-147-56-72
    Average 93 stars, based on 18 article reviews
    martine roussel - by Bioz Stars, 2026-09
    93/100 stars

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    Related Articles

    Generated:

    Article Title: The F-box protein FBXL16 up-regulates the stability of C-MYC oncoprotein by antagonizing the activity of the F-box protein FBW7
    Article Snippet: .. FBXL16ΔLRR was generated by replacing I244 with a stop codon using FBXL16I244Stop-F (ACGCTCAGCGAGGTCTAGCGCGCGCTCAG GC) and its reverse complement. pCDNA3-HA-cmyc was a gift from Martine Roussel (Addgene plasmid #74164 (33)). .. Flag-tagged c-myc was generated by digestion of pCDNA3-HA-c-myc with BamHI and EcoRI restriction enzymes and the insertion of the digested c-myc fragment into pCMVTag2B plasmid containing Flag tag (Stratagene).

    Plasmid Preparation:

    Article Title: The F-box protein FBXL16 up-regulates the stability of C-MYC oncoprotein by antagonizing the activity of the F-box protein FBW7
    Article Snippet: .. FBXL16ΔLRR was generated by replacing I244 with a stop codon using FBXL16I244Stop-F (ACGCTCAGCGAGGTCTAGCGCGCGCTCAG GC) and its reverse complement. pCDNA3-HA-cmyc was a gift from Martine Roussel (Addgene plasmid #74164 (33)). .. Flag-tagged c-myc was generated by digestion of pCDNA3-HA-c-myc with BamHI and EcoRI restriction enzymes and the insertion of the digested c-myc fragment into pCMVTag2B plasmid containing Flag tag (Stratagene).

    Article Title: Optogenetic clustering and membrane translocation of the BcLOV4 photoreceptor.
    Article Snippet: .. ErbB2 was sourced from MSCV- human Erbb2- IRES- GFP, which was a gift from Martine Roussel (Addgene plasmid # 91888; http://n2t.net/ addgene:91888; RRID:Addgene_91888). .. ErbB3 was sourced from pDONR223ERBB3, which was a gift from William Hahn & David Root (Addgene plasmid # 23874; http://n2t.net/addgene:23874; RRID:Addgene_23874).

    Article Title: Emerin is an effector of oncogenic KRAS-driven nuclear dynamics in pancreatic cancer
    Article Snippet: Primers were obtained from IDT (WT = 349 bp, Emd +/– = 490/349 bp, Emd –/– = 490 bp). .. Empty vector, pcDNA-HA2 was a gift from Scott Kaufmann (Division of Oncology Research, Department of Oncology, Mayo Clinic). pcDNA- CYCLIN D1 -T286A-HA was a gift from Bruce Zetter (Vascular Biology and Department of Surgery, Children’s Hospital, Harvard Medical School, Boston, Massachusetts, USA) (RRID: Addgene 11182) ( ). cMyc plasmid, pCDNA3-HA-HA-human CMYC , was a gift from Martine Roussel (Department of Tumor Cell Biology, St. Jude Children’s Research Hospital, Memphis, Tennessee, USA) (RRID: Addgene 74164) ( ). .. Cells were lysed in modified RIPA buffer (50 mM Tris-HCl [pH 7.5] [Invitrogen, 15506-017], 1.0% NP-40 [Fluka BioChemika ,74385], 150 mM NaCl [Sigma-Aldrich, S9625], 5 mM EDTA [Life Technologies, AM9261], 0.25% sodium deoxycholate detergent [Sigma-Aldrich, D6750], and 1.0% SDS [Bio-Rad, 239753]) supplemented with phosphatase inhibitors (Thermo Fisher Scientific, PI78420) and cOmplete protease inhibitor cocktail (Roche, 11836145001).

    Article Title: Early transcriptional and epigenetic divergence of CD8+ T cells responding to acute versus chronic infection.
    Article Snippet: Single-Cell RNA libraries were prepared according to the 10x Genomics Chromium Single-Cell 30 Reagent Kits v2 User Guide or Next GEM Single Cell 30 Reagent kits v3.1 User Guide and sequenced on a HiSeq 4000 (Illumina). .. The MSCV-mouse-Ezh2-IRES-GFP (Ezh2 overexpression, Ezh2-OE) vector was a gift from Martine Roussel (Addgene plasmid #107146; http://n2t.net/addgene:107146; RRID:Addgene 107146). .. To generate retroviral particles, Platinum-E (Plat-E) cells were plated in 10 cm plates 1 day prior to transfection and transfected with 10 μg of the Ezh2-OE or empty vector and 5 μg of pCL-Eco using TransIT-LTI (Mirus).

    Article Title: Lentiviral-Driven Discovery of Cancer Drug Resistance Mutations
    Article Snippet: The HIV reverse transcriptase (RT)–M230I mutant (M-RT) was generated by sitedirected mutagenesis of codon 230 (ATG!ATC) in the RT in the plasmid pCMV-dR8.91 with the Q5 Site-Directed Mutagenesis Kit (New England BioLabs, catalog no. E0554S). .. The BCR-ABL1 open reading frame was PCR amplified from the plasmid MSCV-(pBabemcs)-humanp210BCR-ABL-IRES-GFP that was a gift from Martine Roussel (Addgene plasmid # 79248; RRID: Addgene_79248), and the position of deleted nucleotides in this open reading frame for SV8 and SV11 variants can be found in Supplementary Table S6. ..

    other:

    Article Title: LEFTY1 Is a Dual-SMAD Inhibitor that Promotes Mammary Progenitor Growth and Tumorigenesis.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Software and Algorithms ImageJ ImageJ RRID:SCR_003070 GraphPad Prism GraphPad Software RRID:SCR_002798 CopyCaller software v2.1 Thermo Fisher Scientific https://www.thermofisher.com/us/en/ home/technical-resources/softwaredownloads/copycaller-software.html MATLAB (version 7.3.0.267 (R2006b) MathWorks RRID:SCR_001622 R v3.6.0 RStudio RRID:SCR_000432 ELDA: extreme limiting dilution analysis Webtool http://bioinf.wehi.edu.au/software/elda/

    Article Title: LEFTY1 Is a Dual-SMAD Inhibitor that Promotes Mammary Progenitor Growth and Tumorigenesis.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Recombinant EGF Thermo Fisher Scientific Cat# PMG8043 Recombinant Noggin R&D Systems Cat# 6057-NG Growth Factor Reduced Matrigel BD Biociences Cat# 356230 Matrigel BD Biociences Cat# 356234 LEFTY-1 R&D Systems Cat# 994-LF BMP7 R&D Systems Cat# 5666-BP BMP2 R&D Systems Cat# 355-BM BMP4 R&D Systems Cat# 314-BP CRIPTO-1 R&D Systems Cat# 1538-CR NODAL R&D Systems Cat# 1315-ND Recombinant Human TGF-beta 1 Protein R&D Systems Cat#240-B-002 DAPI Sigma-Aldrich Cat# 32670 Dispase StemCell Technologies Cat#07913 Collagenase/Hyaluronidase StemCell Technologies Cat #07912 DNase I solution StemCell Technologies Cat#07900 Lefty Blocking Peptide Santa Cruz Biotechnolgy Cat# sc-365845 P LDN-193189 Selleckchem Cat# S2618 Critical Commercial Assays Dual-Luciferase Reporter Assay System Promega Cat#E1910 DUOLink Sigma Cat#921021 Pierce CO-IP Kit Thermo Fisher Scientific Cat#26147 Experimental Models: Cell Lines HEK293T ATCC Cat# CRL-3216, RRID:CVCL_0063 MDA-MD-157 ATCC Cat# HTB-24, RRID:CVCL_0618 3T3-L1 feeder cells Kindly provided by Dr. Nusse N/A L1-wnt3a feeder cells Kindly provided by Dr. Nusse N/A MDA-MB-231 ATCC Cat# HTB-26, RRID:CVCL_0062 COMMA-D beta cells Kindly provided by Dr. Medina Campbell et al., 1988 Experimental Models: Organisms/Strains C57BL/6 mice Jackson Laboratory Cat# JAX:000664, RRID:IMSR_JAX:000664 NOD.Cg-Prkdcscid Il2rgtm1Wjl/SzJ (NSG) Jackson Laboratory Cat# JAX:005557, RRID:IMSR_JAX:005557 pCx-GFP Kind gift of Dr. Irving Weissman N/A Oligonucleotides Cloning primers This paper Table S6 qRT-PCR primers This paper Table S6 Copy number determination primers This paper Table S6 Single cell assay ID This paper Table S3 Recombinant DNA pEIZ-HIV-ZsGreen Kind gift of Dr. Zena Werb N/A HIV-Lefty1-ZsG This paper N/A HIV-Che Shimono et al., 2009 N/A HIV-Bmp7-Che This paper N/A pGL3 BRE-luciferase Kind gift of Martine Roussel and Peter ten Dijike, Addgene #45126 pRL-TK Renilla luciferase vector Promega Cat# E2261 pEGFP-C3 (discontinued) Clontech Clontech Bmp7 cDNA clone Origene Cat# MC201085 clone pSICO-R Addgene Cat#12084 Lefty1 cDNA clone IMAGE Cat# 5221120 plasmid clone (Continued on next page) e2 Cell Stem Cell 27, 1–16.e1–e8, August 6, 2020

    Over Expression:

    Article Title: Early transcriptional and epigenetic divergence of CD8+ T cells responding to acute versus chronic infection.
    Article Snippet: Single-Cell RNA libraries were prepared according to the 10x Genomics Chromium Single-Cell 30 Reagent Kits v2 User Guide or Next GEM Single Cell 30 Reagent kits v3.1 User Guide and sequenced on a HiSeq 4000 (Illumina). .. The MSCV-mouse-Ezh2-IRES-GFP (Ezh2 overexpression, Ezh2-OE) vector was a gift from Martine Roussel (Addgene plasmid #107146; http://n2t.net/addgene:107146; RRID:Addgene 107146). .. To generate retroviral particles, Platinum-E (Plat-E) cells were plated in 10 cm plates 1 day prior to transfection and transfected with 10 μg of the Ezh2-OE or empty vector and 5 μg of pCL-Eco using TransIT-LTI (Mirus).

    Polymerase Chain Reaction:

    Article Title: Lentiviral-Driven Discovery of Cancer Drug Resistance Mutations
    Article Snippet: The HIV reverse transcriptase (RT)–M230I mutant (M-RT) was generated by sitedirected mutagenesis of codon 230 (ATG!ATC) in the RT in the plasmid pCMV-dR8.91 with the Q5 Site-Directed Mutagenesis Kit (New England BioLabs, catalog no. E0554S). .. The BCR-ABL1 open reading frame was PCR amplified from the plasmid MSCV-(pBabemcs)-humanp210BCR-ABL-IRES-GFP that was a gift from Martine Roussel (Addgene plasmid # 79248; RRID: Addgene_79248), and the position of deleted nucleotides in this open reading frame for SV8 and SV11 variants can be found in Supplementary Table S6. ..

    Amplification:

    Article Title: Lentiviral-Driven Discovery of Cancer Drug Resistance Mutations
    Article Snippet: The HIV reverse transcriptase (RT)–M230I mutant (M-RT) was generated by sitedirected mutagenesis of codon 230 (ATG!ATC) in the RT in the plasmid pCMV-dR8.91 with the Q5 Site-Directed Mutagenesis Kit (New England BioLabs, catalog no. E0554S). .. The BCR-ABL1 open reading frame was PCR amplified from the plasmid MSCV-(pBabemcs)-humanp210BCR-ABL-IRES-GFP that was a gift from Martine Roussel (Addgene plasmid # 79248; RRID: Addgene_79248), and the position of deleted nucleotides in this open reading frame for SV8 and SV11 variants can be found in Supplementary Table S6. ..



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    93
    Addgene inc martine roussel
    Martine Roussel, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/martine+roussel/pCDNA3-HA-HA-humanCMYC+(Plasmid+%2374164)/pmc12288966-147-56-72
    Average 93 stars, based on 1 article reviews
    martine roussel - by Bioz Stars, 2026-09
    93/100 stars
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